Calcium channels activated by depletion of internal calcium stores in A431 cells
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منابع مشابه
Calcium Current Activated by Depletion of Calcium Stores in Xenopus Oocytes
Ca(2+) currents activated by depletion of Ca(2+) stores in Xenopus oocytes were studied with a two-electrode voltage clamp. Buffering of cytosolic Ca(2+) with EGTA and MeBAPTA abolished I(Cl(Ca)) and unmasked a current in oocytes that was activated by InsP(3) or ionomycin in minutes and by thapsigargin or the chelators themselves over hours. At -60 mV in 10 mM extracellular CaCl(2), the current...
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Yu, B.-Z., Ghomashchi, F., Cajal, Y., Annand, R. A., Berg, 0. G., Gelb, M. H. and Jain, M. H. (1997) Biochemistry 36, 3870-3881 Bayburt, T., Yu, B. Z., Lin, H. K., Browning, J., Jain, M. K. and Gelb, M. H. (1993) Biochemistry 32, 573-582 Scott, D. L., Mandel, A. M., Sigler, P. B. and Honig, B. (1994) Biophys. J. 67, 493-504 Snitko, Y., Koduri, R. S., Han, S. K., Othman, R., Baker, S. F., Molini...
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We have investigated the effects of Ca2+ on Na+ influx through ATP-activated channels in pheochromocytoma PC12 cells using single channel current recordings. Under cell-attached patch-clamp conditions with 150 mM Na+ and 2 mM Ca2+ in the pipette, the unitary current activity showed an open level of about -4.3 pA at -150 mV. The channel opening was interrupted by flickery noise as well as occasi...
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The mechanisms of cellular lead uptake were characterized using a fluorescence method in cells loaded with indo-1. Pb2+ bound to intracellular indo-1 with much higher affinity than Ca2+ and quenched fluorescence at all wavelengths. Pb2+ uptake into pituitary GH3 cells, glial C6 cells, and a subclone of HEK293 cells was assessed by fluorescence quench at a Ca2+-insensitive emission wavelength. P...
متن کاملActivation of calcium entry in human carcinoma A431 cells by store depletion and phospholipase C- dependent mechanisms converge on ICRAC-like calcium channels.
Activation of phospholipase C in nonexcitable cells causes the release of calcium (Ca2+) from intracellular stores and activation of Ca2+ influx by means of Ca2+ release-activated channels (ICRAC) in the plasma membrane. The molecular identity and the mechanism of ICRAC channel activation are poorly understood. Using the patch-clamp technique, here we describe the plasma membrane Ca2+ channels ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1994
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(94)80467-9